Their biotechnological applications are expected to improve the quality of crops while maintaining ecologically and. The beneficial fungus piriformospora indica protects arabidopsis from verticillium dahliae infection by downregulation plant defense responses chao sun1, yongqi shao2, khabat vahabi1, jing lu2,6, samik bhattacharya2, sheqin dong3, kaiwun yeh4, irena sherameti1, binggan lou5, ian t baldwin2 and ralf oelmuller1 abstract. Use features like bookmarks, note taking and highlighting while reading piriformospora indica. Piriformospora indica strains in which the pitam1 gene was silenced via an rna. This copy of the genome of piriformospora indica was obtained from alga zuccaro at the mpi fur terrestrische mikrobiologie in order to allow comparative analyses with other fungal genomes sequenced by the joint genome institute, a copy of this genome is incorporated into mycocosm. Piriformospora indica reduces fusarium head blight disease. Piriformospora indica released factors and its role in the.
In many settings colonisation promotes host growth, increases yield and protects the host from fungal diseases. Characterization of piriformospora indica culture filtrate. Piriformospora indica root colonization triggers local and. The endophyte piriformospora indica colonizes roots of a range of host plants and increases. Sebacinales and their biotechnological applications soil. Verticillium dahliae vd is a soilborne vascular pathogen which causes severe wilt symptoms in a wide range of plants. Plant root epidermal cells are also infected by the plant growthpromoting fungus piriformospora indica basidiomycota, raising the. Sep 17, 2015 arbuscular mycorrhiza am fungi glomeromycota form symbiosis with and deliver nutrients via the roots of most angiosperms.
Kogel institute of phytopathology and applied zoology, research centre for biosystems, land use and nutrition, justusliebiguniversitat gie. Functional characterization of an effector candidate of. The root endophytic fungus piriformospora indica requires host cell death for proliferation during mutualistic symbiosis with barley. Neighbor joining nj phylogenetic tree of 98 concatenated single copy.
The beneficial fungus piriformospora indica protects. Besides this direct beneficial interaction, autoclaved cellwall extracts cwe as well as the application of. It is able to increase biomass and grain yield of crop plants. To investigate systemic effects within the root system, we established a hydroponic splitroot. The process of p solubilisation was caused most likely by proton extrusion and. The aim of the present study is to evaluate the effect of p. Piriformospora indica verma, varma, rexer, kost et. Piriformospora indica and plants dissertation zur erlangung des doktorgrades dr.
Piriformospora indica has been optimized and tested for its growth enhancement in test plants. Recently a cultivable endophytic fungus piriformospora indica fig. Owing to its exceptional ability to efficiently promote plant growth, protection and stress tolerance, a mycorrhiza like endophytic agaricomycetes fungus piriformospora indica has received a great attention over the last few decades. Pdf a new fungus isolate was discovered in an arbuscular mycorrhizal fungal spore from a desert soil in india.
Development of piriformospora indica based formulation. Piriformospora indica, a root endophytic fungus from the sebacinaceae family, has been described to be beneficial for plant growth farkya et al. General introduction 1 general introduction piriformospora indica is a root colonizing endophytic fungi, belonging to the order sebacinales phylum basidiomycota, isolated by verma and collaborators from the rhizosphere of prosopsis juliflora and zizyphus mummularia plants in thar desert, india1. Due to its ease of culture, this fungus provides a model organism for the study of beneficial plantmicrobe interactions and a new tool for improving plant. Serendipita indica root endophyte fungus piriformospora. Research paper piriformospora indica antagonizes cyst. Indian journal of biot chnology vol i, october 2002, pp 372376 piriformospora indica in vitro raised leguminous plants. Pdf the root endophyte piriformospora indicabelongs to the. In the current study, wildtype and transgenic tobacco constitutively overexpressing arabidopsis aquaporins pip 1. Effects of the endophyte piriformospora indica on growth, physiology and water relations of tobacco nicotiana tobacum author creator ferster, frances g.
Piriformospora indica is a widehost rootcolonizing endophytic fungus which allows the plants to grow under extreme physical and nutrient stress. Their biotechnological applications are expected to improve the quality of crops while maintaining ecologically and economically sustainable production systems. Previous studies have shown that the endophytic fungus piriformospora indica promotes the growth and early flowering in coleus forskohlii a medicinal plant and arabidopsis. Research article open access the beneficial fungus.
The fungus can be cultivated on complex and minimal substrates. Am fungal hyphae are taken up by living root epidermal cells, a program which relies on a set of plant common symbiosis genes csgs. There are no page or colour charges and a pdf version will be provided for each. Standardization and evaluation of synthetic media for. Mar 22, 2016 piriformospora indicaplant mutualistic interaction and the role of calcium ions. Biological control of fusarium diseases of wheat by. Piriformospora indica sebacinaceae is a cultivable root endophytic fungus. Piriformospora indica, a cultivable plantgrowthpromoting.
Download it once and read it on your kindle device, pc, phones or tablets. Piriformospora indica is a root endophyte belonging to. Optimization of piriformospora indica the cultures were revived and optimized to grow in laboratory conditions. This thesis reports the effect of piriformospora indica on fusarium diseases of wheat, both head blight and crown rot, with the purpose of developing a solution to control crop diseases by using natural microorganisms. Piriformospora indica is a basidiomycete fungus colonizing roots of a wide range of higher plants, including crop plants and the model plant arabidopsis thaliana.
The fungus lacks host specificity and is cosmopolitan in. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Performance of the biocontrol fungus piriformospora indica on. May 29, 2012 piriformospora indica, a member of the newly created order sebacinales, is extremely versatile in its mycorrhizal associations and its ability to promote plant growth. Flowering in plants is synchronized by both environmental cues and internal regulatory factors. Piriformospora indica strains in which the pitam1 gene was silenced via an rna interference rnai approach were compromised in iaa and ila production and displayed reduced colonization of barley hordeum vulgare roots in the biotrophic phase, but the elicitation of growth promotion was not affected compared with the wild. Piriformospora indica promotes growth, seed yield and. Arbuscular mycorrhiza am fungi glomeromycota form symbiosis with and deliver nutrients via the roots of most angiosperms. Here we demonstrate that an exudate preparation induces cytoplasmic calcium elevation in arabidopsis roots, and the disease development requires the ethyleneactivated. Colonisation of oncidium orchid roots by the endophyte. Both mutualists and commensals are identical for many fungi during the initial phases of infection and colonization by pathogens rodriguez et al. Piriformospora indica is a root endophyte belonging to the sebacinaceae sebacinales, basidiomycota. The fungus has typical pearshaped chlamydospores, and thus, named. Piriformospora indica pi, originally isolated from the woody shrubs.
Ajit verma and group, school of life sciences, jawaharlal nehru university, new delhi. Piriformospora indica hymenomycetes, basidiomycota is a newly described cultivable endophyte that colonizes roots. Our study reveals that the mutualistic symbiont piriformospora indica possesses. Indole derivative production by the root endophyte. Sebacina vermifera promotes the growth and fitness.
Novel cultivable mycobiont piriformospora indica as plant. In this study, pihog1 gene of the root endophytic fungus p. It is a monotypic genus, containing the single endophytic rootcolonising species piriformospora indica, discovered from orchid plants in the thar desert in rajasthan, india by prof. With this extraordinary diversity, sebacinales with the key fungus piriformospora indica might possess remarkable significance in natural ecosystems.
Inoculation with the fungus and application of fungal culture filtrate promotes plant growth and biomass production. Thus, the mode of recognition and early signaling processes are crucial in understanding how plants can differentiate between a beneficial and a detrimental. A new dimension in establishment and phytopromotion archana singh 1, anjana singh 2 and aj it varma 2 i department of biological sciences, michigan technological university, 1400 townsend drive, houghton, michigan 49931, usa. Piriformospora indica, a mycorrhizallike fungus able to establish associations with roots of a wide range of plants, supporting plant nutrition and increasing plant resistance and tolerance to stress, was shown to solubilise phosphate applied in the form of animal bone char habo in fermentation systems. The axenically cultivable root endophyte piriformospora indica is a model organism of this fungal order. The ligation reaction was set up as described in the manual ratio 3. Standardization and evaluation of synthetic media for mass cultivation of the fungus p. A unique endophytic fungus, piriformospora indica, isolated from the hot thar desert, shows beneficial symbiotic associations with a wide range of host plants. The piriformospora indica effector piin 08944 promotes the. The endomycorrhizal fungus piriformospora indica is well known for inducing disease resistance, elevation of salt tolerance and increasing biomass in symbiotic plants.
Detection and characterization of secreted proteins from. Production of a potential liquid plant biostimulant by. The microsclerotia produced by the pathogen survive in soil for more than 15 years. Hybridizationand washing steps were performed at 65. Piriformospora indica and sebacina vermifera increase growth performance at the expense of herbivore resistance in nicotiana attenuata. We thus propose that piriformospora be merged with. Piriformospora indica, a cultivable root endophyte with. Piriformospora indica protects barley from root rot caused.
During the symbiotic interaction, it has also been observed that p. Other names i aftolid 612 achlamydospora indica achlamydospora indicum cbs 125645 dsm 11827 more. In cocultivation experiments in pots for 110 days, plants bolting and flowering were observed 8 days and 9 days earlier as compared to the controls, respectively fig. It colonises the roots of a wide range of host plants. Jun 29, 1998 piriformospora indica hymenomycetes, basidiomycota is a newly described cultivable endophyte that colonizes roots. Effect of piriformospora indica on antioxidant enzymes. Here we demonstrate that an exudate preparation induces cytoplasmic calcium elevation in arabidopsis roots, and the disease development requires the ethyleneactivated transcription factor ein3. Endophytic life strategies decoded by genome and transcriptome. R genes were further analyzed based on manual verification of the. Functions of novel symbiotic fungus piriformospora indica.
Proper characterization of piriformospora indica culture filtrate is an essential prerequisite to understand the underlying and still unknown mechanisms by which this fungus or its culture filtrate acts as a plant growth stimulator, promoter, and bioprotector. Piriformospora indica confers drought tolerance on zea. Cultivable endophytic mycobiont piriformospora indica in. Indole derivative production by the root endophyte piriformospora. Pihog1, a stress regulator map kinase from the root endophyte. The root endophytic fungus piriformospora indica requires. Biotechnological applications of piriformosporaindica. Piriformospora indicaplant mutualistic interaction and the role of calcium ions. Frontiers colonization of root cells and plant growth. Piriformospora indica, a basidiomycete of the sebacinaceae family, was first isolated from bush rhizosphere zones of the thar desert in india. The labeling of the hygromycin b probe was performedusingtheprimeagene r labelingsystemaccording tothemanufacturersinstructionspromega. Map kinase from the root endophyte fungus piriformospora indica.
1538 725 431 1597 690 488 533 720 1457 592 1635 443 633 806 1369 673 1102 620 1461 1633 1473 1513 944 654 248 249 816 1505 416 933 1430 1383 989 976 780 449 1167 545 568 400 321